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Direct solid phase synthesis of peptides and oligonucleotides (ONs) requires high chemical stability of the support material. In this work, we have investigated the passivation ability of porous oxidized silicon multilayered structures by two aminosilane compounds, 3-aminopropyltriethoxysilane and 3-aminopropyldimethylethoxysilane (APDMES), for optical label-free ON biosensor fabrication. We have also studied by spectroscopic reflectometry the hybridization between a 13 bases ON, directly grown on the aminosilane modified porous oxidized silicon by in situ synthesis, and its complementary sequence. Even if the results show that both devices are stable to the chemicals (carbonate/methanol) used, the porous silica structure passivated by APDMES reveals higher functionalization degree due to less steric hindrance of pores.
The Royal Society
Publication date: 
6 Jun 2013

Luca De Stefano, Giorgia Oliviero, Jussara Amato, Nicola Borbone, Gennaro Piccialli, Luciano Mayol, Ivo Rendina, Monica Terracciano, Ilaria Rea

Biblio References: 
Volume: 10 Issue: 83 Pages: 20130160
Journal of the Royal Society Interface